UTGBMedaka/Track: EST_libraries.htm

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6<h1>Medaka EST libraries</h1>
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8<p>
9EST libraries information listed below are based on <a href = "http://www.ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID=8090">Unigne database build#19 </A>in ncbi.<br>
10You can download the libarary information (this table) and the individual EST libray sequences as a multifasta format from our dowlload site.
11</P>
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13<Table Border>
14<Tr>
15<Td>Clone name</Td><Td>RNA source</Td><Td>strain</Td><Td>Vector</Td><Td>Cloning site</Td><Td>Antibiotics</Td><Td>Linker/Adoptor</Td><Td>Sequencing primer for 5' region</Td><Td>Sequencing primer for 3' region</Td><Td>Host strain</Td><Td>Produced by</Td><Td>Reference</Td><Td>Notes</Td>
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18<Td>Ola+</Td><Td>Male and female adult whole body</Td><Td>HNI</Td><Td>pME18s@iAB009864@1193,1194 modified from CC to AT)</Td><Td><i>Dra</i> III</Td><Td>-</Td><Td>5' AGGAUCGAGUCGGCCUUGUUGGCCUAGUGG 3'</Td><Td>-</Td><Td>-</Td><Td>Top10</Td><Td>S.Sugano, K.Kawakami, M.Suzuki ( The Institute of Medical Science The University of Tokyo )</Td><Td>-</Td><Td>Full length library</Td>
19</tr>
20<tr>
21<Td>Olb+</Td><Td>Cultured medaka cell derived from caudal fin of adult male</Td><Td>HNI</Td><Td>pBluescriptSK(+)</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>TCGAAATTAACCCTCACTAAAGGG</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani</Td><Td>-</Td>
22</tr>
23<tr>
24<Td>Olc+</Td><Td>Cultured medaka cell derived from caudal fin of adult male (with 20J/m2 UV } PR)</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>SURE</Td><Td>H.Mitani</Td><Td>-</Td>
25</tr>
26<tr>
27<Td>Old+</Td><Td>Ovary</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani, K.Naruse, M.Kondo</Td><Td>-</Td>
28</tr>
29<tr>
30<Td>Ole+</Td><Td>Liver (female &amp; male)</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani, K.Naruse, M.Kondo</Td><Td>-</Td>
31</tr>
32<tr>
33<Td>Olf+</Td><Td>Cultured medaka cell derived from caudal fin of adult male (with gamma-irradiation)</Td><Td>HNI</Td><Td>pBluescriptSK(+)</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence  (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'  </Td><Td>TCGAAATTAACCCTCACTAAAGGG</Td><Td>-</Td><Td>SURE</Td><Td>H.Mitani</Td><Td>-</Td>
34</tr>
35<tr>
36<Td>SNK01+</Td><Td>Eye</Td><Td>Wild population from Okayama Pref. (Southern part of Japan)</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>-</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>E.Sanaka, H.Hori</Td>
37</tr>
38<tr>
39<Td>Ol-br-ad+</Td><Td>Brain (female &amp; male)</Td><Td>HNI</Td><Td>pBluescriptSK(-)</Td><Td><i>EcoR</i> I, <i>Xho</i> I</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3: </Td><Td>-</Td><Td>-</Td><Td>Mita,K., Ishikawa,Y., Yamauchi,M.</Td><Td>-</Td>
40</tr>
41<tr>
42<Td>olrf+</Td><Td>regenerating fin 3dpa or 10dpa</Td><Td>Cab</Td><Td>-</Td><Td><i>EcoR</i> I, <i>Xho</i> I</Td><Td>Ampicillin</Td><Td>-</Td><Td>BS740: CCGCTCTAGAACTAGTG</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>Katogi,R., Nakatani,Y., Shin-I,T., Kohara,Y., Inohaya,K. and Kudo,A.</Td><Td>Mech. Dev. <b>121</b>, 861-872 (2004)</Td>
43</tr>
44<tr>
45<Td>McF+</Td><Td>gastrula</Td><Td>Cab</Td><Td>pCSII+</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
46</tr>
47<tr>
48<Td>McF+</Td><Td>neurula</Td><Td>Cab</Td><Td>pCSII+</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
49</tr>
50<Tr>
51<Td>FOE002+</Td><Td>stage 18 24 32 36 whole embryo</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Hong,Y., Loosli,F., Ruan,Y., Henrich,T., Wei,C.L., Dallner,C., Wittbrodt,J</Td><Td>Unpublished</Td>
52</tr>
53<tr>
54<Td>McF+</Td><Td>Ovary</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
55</tr>
56<tr>
57<Td>McF+</Td><Td>organogenesis stage</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
58</tr>
59<tr>
60<Td>631-+</Td><Td>Stage 24 </Td><Td>Cab</Td><Td>pSPORT</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>T7: TAATACGACTCACTATAGGG</Td><Td>-</Td><Td>DH5 alpha</Td><Td>J. Wittbrodt, B. Wittbrodt, C. Burgtorf clone request J. Wittbrodt </Td><Td>Nucleic Acids Res. <b>31</b>, 72-74 (2003)</Td></tr>
61<tr>
62<Td>OL30VN+</Td><Td>anterior head of stage 30-31</Td><Td>Orange Red</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Nguyen,V., Joly,J., Bourrat,F.</Td><Td>Mech. Dev. <b>107</b>, 55-67 (2001)</Td>
63</tr>
64<tr>
65<Td>F01SSA+</Td><Td>Stage 20-25 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
66</tr>
67<tr>
68<Td>MF01SSB+</Td><Td>Stage 20-25 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
69</Tr>
70<Tr>
71<Td>MF015DA+</Td><Td>Stage 35 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
72</Tr>
73<Tr>
74<Td>MF01FSA+</Td><Td>Stage 40 whole embryoes</Td><Td>d-rR</Td><Td>pBluescript II SK(-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
75</tr>
76<tr>
77<Td>MF01FFA+</Td><Td>Stage 40 whole embryoes</Td><Td>Hd-rR</Td><Td>pME18SFL3</Td><Td><i>Dra</i> III(808) (5' end), <i>Dra</i> III(1191) (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>pME721F : AACTGCTCCTCAGTGGATGTT</Td><Td>pME1459R : AGGAATTAATTCGAGCTCGGT</Td><Td>-</Td><Td>-</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td><Td>Full length library</Td>
78</tr>
79<tr>
80<td>MF01ASD+</Td><Td>adult female whole body</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Unpublished</Td>
81</tr>
82<tr>
83<Td>MF01ALA+</Td><Td>Adult liver (female &amp; male)</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Unpublished</Td>
84</tr>
85</Table>
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