EST libraries information listed below are based on Unigne database build#19 in ncbi.
You can download the libarary information (this table) and the individual EST libray sequences as a multifasta format from our dowlload site.
| Clone name | RNA source | strain | Vector | Cloning site | Antibiotics | Linker/Adoptor | Sequencing primer for 5' region | Sequencing primer for 3' region | Host strain | Produced by | Reference | Notes |
| Ola+ | Male and female adult whole body | HNI | pME18s （AB009864 1193,1194 modified from CC to AT) | Dra III | - | 5' AGGAUCGAGUCGGCCUUGUUGGCCUAGUGG 3' | - | - | Top10 | S.Sugano, K.Kawakami, M.Suzuki ( The Institute of Medical Science The University of Tokyo ) | - | Full length library |
| Olb+ | Cultured medaka cell derived from caudal fin of adult male | HNI | pBluescriptSK(+) | EcoR I, Sal I (disappeared after cloning) | Ampicillin | oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence 5'-OH-AATTCGGCACGAGG-3' | TCGAAATTAACCCTCACTAAAGGG | - | XL1 Blue MRF' | H.Mitani | - | |
| Olc+ | Cultured medaka cell derived from caudal fin of adult male (with 20J/m2 UV ± PR) | HNI | pUC118 | EcoR I, Sal I (disappeared after cloning) | Ampicillin | oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence 5'-OH-AATTCGGCACGAGG-3' | GGAAACAGCTATGACCATGATTAC | - | SURE | H.Mitani | - | |
| Old+ | Ovary | HNI | pUC118 | EcoR I, Sal I (disappeared after cloning) | Ampicillin | oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence 5'-OH-AATTCGGCACGAGG-3' | GGAAACAGCTATGACCATGATTAC | - | XL1 Blue MRF' | H.Mitani, K.Naruse, M.Kondo | - | |
| Ole+ | Liver (female & male) | HNI | pUC118 | EcoR I, Sal I (disappeared after cloning) | Ampicillin | oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence 5'-OH-AATTCGGCACGAGG-3' | GGAAACAGCTATGACCATGATTAC | - | XL1 Blue MRF' | H.Mitani, K.Naruse, M.Kondo | - | |
| Olf+ | Cultured medaka cell derived from caudal fin of adult male (with gamma-irradiation) | HNI | pBluescriptSK(+) | EcoR I, Sal I (disappeared after cloning) | Ampicillin | oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence 5'-OH-AATTCGGCACGAGG-3' | TCGAAATTAACCCTCACTAAAGGG | - | SURE | H.Mitani | - | |
| SNK01+ | Eye | Wild population from Okayama Pref. (Southern part of Japan) | pUC118 | EcoR I, Sal I (disappeared after cloning) | Ampicillin | - | GGAAACAGCTATGACCATGATTAC | - | XL1 Blue MRF' | E.Sanaka, H.Hori | ||
| Ol-br-ad+ | Brain (female & male) | HNI | pBluescriptSK(-) | EcoR I, Xho I | Ampicillin | - | T3: | - | - | Mita,K., Ishikawa,Y., Yamauchi,M. | - | |
| olrf+ | regenerating fin 3dpa or 10dpa | Cab | - | EcoR I, Xho I | Ampicillin | - | BS740: CCGCTCTAGAACTAGTG | - | XL1 Blue MRF' | Katogi,R., Nakatani,Y., Shin-I,T., Kohara,Y., Inohaya,K. and Kudo,A. | Mech. Dev. 121, 861-872 (2004) | |
| McF+ | gastrula | Cab | pCSII+ | - | - | - | - | - | - | Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H. | Unpublished | |
| McF+ | neurula | Cab | pCSII+ | - | - | - | - | - | - | Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H. | Unpublished | |
| FOE002+ | stage 18 24 32 36 whole embryo | Cab | - | - | - | - | - | - | - | Hong,Y., Loosli,F., Ruan,Y., Henrich,T., Wei,C.L., Dallner,C., Wittbrodt,J | Unpublished | |
| McF+ | Ovary | Cab | - | - | - | - | - | - | - | Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H. | Unpublished | |
| McF+ | organogenesis stage | Cab | - | - | - | - | - | - | - | Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H. | Unpublished | |
| 631-+ | Stage 24 | Cab | pSPORT | - | - | - | T7: TAATACGACTCACTATAGGG | - | DH5 alpha | J. Wittbrodt, B. Wittbrodt, C. Burgtorf clone request J. Wittbrodt | Nucleic Acids Res. 31, 72-74 (2003) | |
| OL30VN+ | anterior head of stage 30-31 | Orange Red | pBluescript II SK (-) | EcoR I (5' end), Xho I (3' end) | - | - | - | - | - | Nguyen,V., Joly,J., Bourrat,F. | Mech. Dev. 107, 55-67 (2001) | |
| F01SSA+ | Stage 20-25 whole embryoes | Hd-rR | pBluescript II SK (-) | EcoR I (5' end), Xho I (3' end) | Ampicillin | - | T3 | T7 | - | Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H. | Mech. Dev. 121, 915-932 (2004) | |
| MF01SSB+ | Stage 20-25 whole embryoes | Hd-rR | pBluescript II SK (-) | EcoR I (5' end), Xho I (3' end) | Ampicillin | - | T3 | T7 | - | Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H. | Mech. Dev. 121, 915-932 (2004) | |
| MF015DA+ | Stage 35 whole embryoes | Hd-rR | pBluescript II SK (-) | EcoR I (5' end), Xho I (3' end) | Ampicillin | - | T3 | T7 | - | Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H. | Mech. Dev. 121, 915-932 (2004) | |
| MF01FSA+ | Stage 40 whole embryoes | d-rR | pBluescript II SK(-) | EcoR I (5' end), Xho I (3' end) | Ampicillin | - | T3 | T7 | - | - | Mech. Dev. 121, 915-932 (2004) | |
| MF01FFA+ | Stage 40 whole embryoes | Hd-rR | pME18SFL3 | Dra III(808) (5' end), Dra III(1191) (3' end) | Ampicillin | - | pME721F : AACTGCTCCTCAGTGGATGTT | pME1459R : AGGAATTAATTCGAGCTCGGT | - | - | Mech. Dev. 121, 915-932 (2004) | Full length library |
| MF01ASD+ | adult female whole body | Hd-rR | pBluescript II SK (-) | EcoR I (5' end), Xho I (3' end) | Ampicillin | - | T3 | T7 | - | - | Unpublished | |
| MF01ALA+ | Adult liver (female & male) | Hd-rR | pBluescript II SK (-) | EcoR I (5' end), Xho I (3' end) | Ampicillin | - | T3 | T7 | - | - | Unpublished |