| 130 | |
| 131 | In addition to the genome of the Hd-rR medaka inbred strain, |
| 132 | the genome of another inbred strain HNI was also sequenced to produce the draft 648-Mb HNI genome. |
| 133 | Inbred strains Hd-rR and HNI originated in the southern and northern Japanese populations, respectively. |
| 134 | They can mate and produce healthy offspring, |
| 135 | although they are estimated to have diverged about 4 million years ago, |
| 136 | and their genome sequences have diverged by approximately 3.42%. |
| 137 | The alignment of the two medaka genomes identified |
| 138 | about 16.4 million single nucleotide polymorphisms (SNPs), from which 2,401 SNPs were selected |
| 139 | and mapped genetically onto medaka chromosomes using a backcross panel between these two inbred strains. |
| 140 | |
| 141 | The alignment of the Hd-rR and HNI genomes revealed 16,519,460 SNPs and 2,859,905 insertions/deletions (indels), |
| 142 | and the locations of individual SNPs and indels are given in the web database. |
| 143 | Pink indicates matched bases, while mismatches are highlighted by green, yellow, blue, and red that represent A, C, G, and T, respectively, in the HNI genome. |
| 144 | Cyan indicates the two possibilities, deletions from the HNI genome or insertions into the Hd-rR genome, |
| 145 | though the settlement requires comparison with the genome of an outgroup of Hd-rR and HNI. |
| 146 | Gray means the failure of the alignment because of low quality bases in either of the two genomes or lack of HNI reads in the region. |
| 147 | |