| | 74 | A total of 142,414 bacterial artificial chromosome (BAC) end sequences are mapped to medaka genome. |
| | 75 | Three types of BAC library are of average size 135, 180, and 210 kb are used. The accession |
| | 76 | numbers of the reference BAC sequences are AB102768, BA000027, AB251575, and AB232918-24, respectively. To reduce |
| | 77 | computation time, the assembly was aligned against each reference BAC using BLAT with the default parameters, and the |
| | 78 | scaffold that was most similar to the reference was selected. The selected scaffold was then aligned to the reference BAC using |
| | 79 | NCBI-BLAST (The parameters were all default, except the e-value threshold was set to 1e-10) |
| | 80 | |
