| 1 |
<html> |
|---|
| 2 |
<body BgColor="lightcyan"> |
|---|
| 3 |
<Basefont Size="5"> |
|---|
| 4 |
</body> |
|---|
| 5 |
|
|---|
| 6 |
<h1>Medaka EST libraries</h1> |
|---|
| 7 |
|
|---|
| 8 |
<p> |
|---|
| 9 |
EST libraries information listed below are based on <a href = "http://www.ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID=8090">Unigne database build#19 </A>in ncbi.<br> |
|---|
| 10 |
You can download the libarary information (this table) and the individual EST libray sequences as a multifasta format from our dowlload site. |
|---|
| 11 |
</P> |
|---|
| 12 |
|
|---|
| 13 |
<Table Border> |
|---|
| 14 |
<Tr> |
|---|
| 15 |
<Td>Clone name</Td><Td>RNA source</Td><Td>strain</Td><Td>Vector</Td><Td>Cloning site</Td><Td>Antibiotics</Td><Td>Linker/Adoptor</Td><Td>Sequencing primer for 5' region</Td><Td>Sequencing primer for 3' region</Td><Td>Host strain</Td><Td>Produced by</Td><Td>Reference</Td><Td>Notes</Td> |
|---|
| 16 |
</tr> |
|---|
| 17 |
<tr> |
|---|
| 18 |
<Td>Ola+</Td><Td>Male and female adult whole body</Td><Td>HNI</Td><Td>pME18s@iAB009864@1193,1194 modified from CC to AT)</Td><Td><i>Dra</i> III</Td><Td>-</Td><Td>5' AGGAUCGAGUCGGCCUUGUUGGCCUAGUGG 3'</Td><Td>-</Td><Td>-</Td><Td>Top10</Td><Td>S.Sugano, K.Kawakami, M.Suzuki ( The Institute of Medical Science The University of Tokyo )</Td><Td>-</Td><Td>Full length library</Td> |
|---|
| 19 |
</tr> |
|---|
| 20 |
<tr> |
|---|
| 21 |
<Td>Olb+</Td><Td>Cultured medaka cell derived from caudal fin of adult male</Td><Td>HNI</Td><Td>pBluescriptSK(+)</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>TCGAAATTAACCCTCACTAAAGGG</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani</Td><Td>-</Td> |
|---|
| 22 |
</tr> |
|---|
| 23 |
<tr> |
|---|
| 24 |
<Td>Olc+</Td><Td>Cultured medaka cell derived from caudal fin of adult male (with 20J/m2 UV } PR)</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>SURE</Td><Td>H.Mitani</Td><Td>-</Td> |
|---|
| 25 |
</tr> |
|---|
| 26 |
<tr> |
|---|
| 27 |
<Td>Old+</Td><Td>Ovary</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani, K.Naruse, M.Kondo</Td><Td>-</Td> |
|---|
| 28 |
</tr> |
|---|
| 29 |
<tr> |
|---|
| 30 |
<Td>Ole+</Td><Td>Liver (female & male)</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani, K.Naruse, M.Kondo</Td><Td>-</Td> |
|---|
| 31 |
</tr> |
|---|
| 32 |
<tr> |
|---|
| 33 |
<Td>Olf+</Td><Td>Cultured medaka cell derived from caudal fin of adult male (with gamma-irradiation)</Td><Td>HNI</Td><Td>pBluescriptSK(+)</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3' </Td><Td>TCGAAATTAACCCTCACTAAAGGG</Td><Td>-</Td><Td>SURE</Td><Td>H.Mitani</Td><Td>-</Td> |
|---|
| 34 |
</tr> |
|---|
| 35 |
<tr> |
|---|
| 36 |
<Td>SNK01+</Td><Td>Eye</Td><Td>Wild population from Okayama Pref. (Southern part of Japan)</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>-</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>E.Sanaka, H.Hori</Td> |
|---|
| 37 |
</tr> |
|---|
| 38 |
<tr> |
|---|
| 39 |
<Td>Ol-br-ad+</Td><Td>Brain (female & male)</Td><Td>HNI</Td><Td>pBluescriptSK(-)</Td><Td><i>EcoR</i> I, <i>Xho</i> I</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3: </Td><Td>-</Td><Td>-</Td><Td>Mita,K., Ishikawa,Y., Yamauchi,M.</Td><Td>-</Td> |
|---|
| 40 |
</tr> |
|---|
| 41 |
<tr> |
|---|
| 42 |
<Td>olrf+</Td><Td>regenerating fin 3dpa or 10dpa</Td><Td>Cab</Td><Td>-</Td><Td><i>EcoR</i> I, <i>Xho</i> I</Td><Td>Ampicillin</Td><Td>-</Td><Td>BS740: CCGCTCTAGAACTAGTG</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>Katogi,R., Nakatani,Y., Shin-I,T., Kohara,Y., Inohaya,K. and Kudo,A.</Td><Td>Mech. Dev. <b>121</b>, 861-872 (2004)</Td> |
|---|
| 43 |
</tr> |
|---|
| 44 |
<tr> |
|---|
| 45 |
<Td>McF+</Td><Td>gastrula</Td><Td>Cab</Td><Td>pCSII+</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td> |
|---|
| 46 |
</tr> |
|---|
| 47 |
<tr> |
|---|
| 48 |
<Td>McF+</Td><Td>neurula</Td><Td>Cab</Td><Td>pCSII+</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td> |
|---|
| 49 |
</tr> |
|---|
| 50 |
<Tr> |
|---|
| 51 |
<Td>FOE002+</Td><Td>stage 18 24 32 36 whole embryo</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Hong,Y., Loosli,F., Ruan,Y., Henrich,T., Wei,C.L., Dallner,C., Wittbrodt,J</Td><Td>Unpublished</Td> |
|---|
| 52 |
</tr> |
|---|
| 53 |
<tr> |
|---|
| 54 |
<Td>McF+</Td><Td>Ovary</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td> |
|---|
| 55 |
</tr> |
|---|
| 56 |
<tr> |
|---|
| 57 |
<Td>McF+</Td><Td>organogenesis stage</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td> |
|---|
| 58 |
</tr> |
|---|
| 59 |
<tr> |
|---|
| 60 |
<Td>631-+</Td><Td>Stage 24 </Td><Td>Cab</Td><Td>pSPORT</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>T7: TAATACGACTCACTATAGGG</Td><Td>-</Td><Td>DH5 alpha</Td><Td>J. Wittbrodt, B. Wittbrodt, C. Burgtorf clone request J. Wittbrodt </Td><Td>Nucleic Acids Res. <b>31</b>, 72-74 (2003)</Td></tr> |
|---|
| 61 |
<tr> |
|---|
| 62 |
<Td>OL30VN+</Td><Td>anterior head of stage 30-31</Td><Td>Orange Red</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Nguyen,V., Joly,J., Bourrat,F.</Td><Td>Mech. Dev. <b>107</b>, 55-67 (2001)</Td> |
|---|
| 63 |
</tr> |
|---|
| 64 |
<tr> |
|---|
| 65 |
<Td>F01SSA+</Td><Td>Stage 20-25 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td> |
|---|
| 66 |
</tr> |
|---|
| 67 |
<tr> |
|---|
| 68 |
<Td>MF01SSB+</Td><Td>Stage 20-25 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td> |
|---|
| 69 |
</Tr> |
|---|
| 70 |
<Tr> |
|---|
| 71 |
<Td>MF015DA+</Td><Td>Stage 35 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td> |
|---|
| 72 |
</Tr> |
|---|
| 73 |
<Tr> |
|---|
| 74 |
<Td>MF01FSA+</Td><Td>Stage 40 whole embryoes</Td><Td>d-rR</Td><Td>pBluescript II SK(-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td> |
|---|
| 75 |
</tr> |
|---|
| 76 |
<tr> |
|---|
| 77 |
<Td>MF01FFA+</Td><Td>Stage 40 whole embryoes</Td><Td>Hd-rR</Td><Td>pME18SFL3</Td><Td><i>Dra</i> III(808) (5' end), <i>Dra</i> III(1191) (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>pME721F : AACTGCTCCTCAGTGGATGTT</Td><Td>pME1459R : AGGAATTAATTCGAGCTCGGT</Td><Td>-</Td><Td>-</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td><Td>Full length library</Td> |
|---|
| 78 |
</tr> |
|---|
| 79 |
<tr> |
|---|
| 80 |
<td>MF01ASD+</Td><Td>adult female whole body</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Unpublished</Td> |
|---|
| 81 |
</tr> |
|---|
| 82 |
<tr> |
|---|
| 83 |
<Td>MF01ALA+</Td><Td>Adult liver (female & male)</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Unpublished</Td> |
|---|
| 84 |
</tr> |
|---|
| 85 |
</Table> |
|---|
| 86 |
</html> |
|---|
