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3 | <Basefont Size="5">
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4 | </body>
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5 |
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6 | <h1>Medaka EST libraries</h1>
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7 |
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8 | <p>
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9 | EST libraries information listed below are based on <a href = "http://www.ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID=8090">Unigne database build#19 </A>in ncbi.<br>
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10 | You can download the libarary information (this table) and the individual EST libray sequences as a multifasta format from our dowlload site.
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11 | </P>
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12 |
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13 | <Table Border>
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14 | <Tr>
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15 | <Td>Clone name</Td><Td>RNA source</Td><Td>strain</Td><Td>Vector</Td><Td>Cloning site</Td><Td>Antibiotics</Td><Td>Linker/Adoptor</Td><Td>Sequencing primer for 5' region</Td><Td>Sequencing primer for 3' region</Td><Td>Host strain</Td><Td>Produced by</Td><Td>Reference</Td><Td>Notes</Td>
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16 | </tr>
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17 | <tr>
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18 | <Td>Ola+</Td><Td>Male and female adult whole body</Td><Td>HNI</Td><Td>pME18s@iAB009864@1193,1194 modified from CC to AT)</Td><Td><i>Dra</i> III</Td><Td>-</Td><Td>5' AGGAUCGAGUCGGCCUUGUUGGCCUAGUGG 3'</Td><Td>-</Td><Td>-</Td><Td>Top10</Td><Td>S.Sugano, K.Kawakami, M.Suzuki ( The Institute of Medical Science The University of Tokyo )</Td><Td>-</Td><Td>Full length library</Td>
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19 | </tr>
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20 | <tr>
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21 | <Td>Olb+</Td><Td>Cultured medaka cell derived from caudal fin of adult male</Td><Td>HNI</Td><Td>pBluescriptSK(+)</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>TCGAAATTAACCCTCACTAAAGGG</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani</Td><Td>-</Td>
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22 | </tr>
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23 | <tr>
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24 | <Td>Olc+</Td><Td>Cultured medaka cell derived from caudal fin of adult male (with 20J/m2 UV } PR)</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>SURE</Td><Td>H.Mitani</Td><Td>-</Td>
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25 | </tr>
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26 | <tr>
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27 | <Td>Old+</Td><Td>Ovary</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani, K.Naruse, M.Kondo</Td><Td>-</Td>
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28 | </tr>
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29 | <tr>
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30 | <Td>Ole+</Td><Td>Liver (female & male)</Td><Td>HNI</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3'</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>H.Mitani, K.Naruse, M.Kondo</Td><Td>-</Td>
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31 | </tr>
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32 | <tr>
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33 | <Td>Olf+</Td><Td>Cultured medaka cell derived from caudal fin of adult male (with gamma-irradiation)</Td><Td>HNI</Td><Td>pBluescriptSK(+)</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18Eadaptor sequence 5'-OH-AATTCGGCACGAGG-3' </Td><Td>TCGAAATTAACCCTCACTAAAGGG</Td><Td>-</Td><Td>SURE</Td><Td>H.Mitani</Td><Td>-</Td>
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34 | </tr>
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35 | <tr>
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36 | <Td>SNK01+</Td><Td>Eye</Td><Td>Wild population from Okayama Pref. (Southern part of Japan)</Td><Td>pUC118</Td><Td><i>EcoR</i> I, <i>Sal</i> I (disappeared after cloning)</Td><Td>Ampicillin</Td><Td>-</Td><Td>GGAAACAGCTATGACCATGATTAC</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>E.Sanaka, H.Hori</Td>
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37 | </tr>
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38 | <tr>
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39 | <Td>Ol-br-ad+</Td><Td>Brain (female & male)</Td><Td>HNI</Td><Td>pBluescriptSK(-)</Td><Td><i>EcoR</i> I, <i>Xho</i> I</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3: </Td><Td>-</Td><Td>-</Td><Td>Mita,K., Ishikawa,Y., Yamauchi,M.</Td><Td>-</Td>
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40 | </tr>
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41 | <tr>
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42 | <Td>olrf+</Td><Td>regenerating fin 3dpa or 10dpa</Td><Td>Cab</Td><Td>-</Td><Td><i>EcoR</i> I, <i>Xho</i> I</Td><Td>Ampicillin</Td><Td>-</Td><Td>BS740: CCGCTCTAGAACTAGTG</Td><Td>-</Td><Td>XL1 Blue MRF'</Td><Td>Katogi,R., Nakatani,Y., Shin-I,T., Kohara,Y., Inohaya,K. and Kudo,A.</Td><Td>Mech. Dev. <b>121</b>, 861-872 (2004)</Td>
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43 | </tr>
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44 | <tr>
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45 | <Td>McF+</Td><Td>gastrula</Td><Td>Cab</Td><Td>pCSII+</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
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46 | </tr>
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47 | <tr>
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48 | <Td>McF+</Td><Td>neurula</Td><Td>Cab</Td><Td>pCSII+</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
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49 | </tr>
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50 | <Tr>
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51 | <Td>FOE002+</Td><Td>stage 18 24 32 36 whole embryo</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Hong,Y., Loosli,F., Ruan,Y., Henrich,T., Wei,C.L., Dallner,C., Wittbrodt,J</Td><Td>Unpublished</Td>
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52 | </tr>
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53 | <tr>
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54 | <Td>McF+</Td><Td>Ovary</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
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55 | </tr>
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56 | <tr>
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57 | <Td>McF+</Td><Td>organogenesis stage</Td><Td>Cab</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Berger,A., Dohm,J.C., Hennig,S., Sasaki,T., Furutani-Seiki ,M., Mitani,H., Shima,A., Lehrach,H., Wittbrodt,J., Kondoh ,H., Shimizu,N., Himmelbauer,H.</Td><Td>Unpublished</Td>
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58 | </tr>
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59 | <tr>
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60 | <Td>631-+</Td><Td>Stage 24 </Td><Td>Cab</Td><Td>pSPORT</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>T7: TAATACGACTCACTATAGGG</Td><Td>-</Td><Td>DH5 alpha</Td><Td>J. Wittbrodt, B. Wittbrodt, C. Burgtorf clone request J. Wittbrodt </Td><Td>Nucleic Acids Res. <b>31</b>, 72-74 (2003)</Td></tr>
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61 | <tr>
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62 | <Td>OL30VN+</Td><Td>anterior head of stage 30-31</Td><Td>Orange Red</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>-</Td><Td>Nguyen,V., Joly,J., Bourrat,F.</Td><Td>Mech. Dev. <b>107</b>, 55-67 (2001)</Td>
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63 | </tr>
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64 | <tr>
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65 | <Td>F01SSA+</Td><Td>Stage 20-25 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
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66 | </tr>
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67 | <tr>
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68 | <Td>MF01SSB+</Td><Td>Stage 20-25 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
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69 | </Tr>
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70 | <Tr>
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71 | <Td>MF015DA+</Td><Td>Stage 35 whole embryoes</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T. and Takeda ,H.</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
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72 | </Tr>
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73 | <Tr>
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74 | <Td>MF01FSA+</Td><Td>Stage 40 whole embryoes</Td><Td>d-rR</Td><Td>pBluescript II SK(-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td>
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75 | </tr>
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76 | <tr>
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77 | <Td>MF01FFA+</Td><Td>Stage 40 whole embryoes</Td><Td>Hd-rR</Td><Td>pME18SFL3</Td><Td><i>Dra</i> III(808) (5' end), <i>Dra</i> III(1191) (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>pME721F : AACTGCTCCTCAGTGGATGTT</Td><Td>pME1459R : AGGAATTAATTCGAGCTCGGT</Td><Td>-</Td><Td>-</Td><Td>Mech. Dev. <b>121</b>, 915-932 (2004)</Td><Td>Full length library</Td>
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78 | </tr>
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79 | <tr>
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80 | <td>MF01ASD+</Td><Td>adult female whole body</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Unpublished</Td>
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81 | </tr>
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82 | <tr>
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83 | <Td>MF01ALA+</Td><Td>Adult liver (female & male)</Td><Td>Hd-rR</Td><Td>pBluescript II SK (-)</Td><Td><i>EcoR</i> I (5' end), <i>Xho</i> I (3' end)</Td><Td>Ampicillin</Td><Td>-</Td><Td>T3</Td><Td>T7</Td><Td>-</Td><Td>-</Td><Td>Unpublished</Td>
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84 | </tr>
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85 | </Table>
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